Protease activity in single-chain prekallikrein
نویسندگان
چکیده
منابع مشابه
Activity of two-chain recombinant human cytomegalovirus protease.
The human cytomegalovirus UL80 protease was expressed in Escherichia coli and purified by metal-chelate chromatography using a histidine tag engineered at the amino terminus. Cleavage of the 30-kDa protease at an internal site, VEA/A144, resulted in the recovery of 16- plus 14-kDa two-chain protease. The amino-terminal 16-kDa chain and the carboxyl-terminal 14-kDa chain remained associated as a...
متن کاملPrekallikrein Deficiency in Man
Blood plasma obtained from an individual with abnormal thromboplastin formation, due to deficiency of Fletcher factor, was fully corrected by 2% of normal, Hageman factor- or PTA-deficient plasma. It was also reconstituted by addition of highly purified human or rabbit prekallikrein. The plasma failed to generate kinin upon exposure to kaolin, a defect which was also corrected by addition of pr...
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We describe a FRET-based protease detection strategy, using a single-fluorescent-protein nanogel as donor and a dark quencher as acceptor linked by a photolabile caged-peptide. This design enables probing of protease activity in a UV-responsive fashion.
متن کاملPrekallikrein (Fletcher factor) deficiency.
One new case and 29 reported cases of hereditary prekallikrein (Fletcher factor) deficiency are reviewed. Abnormalities in the coagulation, fibrinolytic, complement, and kinin systems are described. These cases are discovered incidentally by prolonged partial thromboplastin times (PTTs) which correct with extended incubation in the presence of a contact activator. Prekallikrein levels are less ...
متن کاملA Prealbumin Activator of Prekallikrein
Activation of a plasma fraction containing unactivated Hageman factor and prekallikrein followed by chromatography of this fraction on DEAE-cellulose revealed four peaks having bradykinin-generating activity. Peak 1 contained kallikrein; peaks 2-3, 4, and 5 each contained prekallikrein-activating activity. Elution of peaks 2-3, 4, and 5 from disc gels after electrophoresis at pH 9.3 revealed pe...
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ژورنال
عنوان ژورنال: Blood
سال: 2020
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.2019002224